Screening for Recombinant E. coli Using Antibiotic Test Strips
نویسندگان
چکیده
منابع مشابه
Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
متن کاملOptimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
متن کاملRapid determination of antibiotic resistance in E. coli using dielectrophoresis.
In recent years, infections due to antibiotic-resistant strains of bacteria such as methicillin-resistant Staphylococcus aureus and ciprofloxacin-resistant Escherichia coli are on the rise, and with them the demand for rapid antibiotic testing is also rising. Conventional tests, such as disc diffusion testing, require a primary sample to be tested in the presence of a number of antibiotics to v...
متن کاملNew Test System for Serine/Threonine Protein Kinase Inhibitors Screening: E. coli APHVIII/Pk25 design.
An efficient test system for serine/threonine protein kinase inhibitors screening has been developed based on theE. coliprotein system APHVIII/Pk25. Phosphorylation of aminoglycoside phosphotransferase VIII (APHVIII) by protein kinases enhances resistance of the bacterial cell to aminoglycoside antibiotics, e.g. kanamycin. Addition of protein kinase inhibitors prevents phosphorylation and incre...
متن کاملUrine Test Strips
Procedure and Notes • Use only well mixed, non-centrifuged urine, which should not be older than 4 hours. First morning urine is recommended. Protect the samples from light. • If the samples cannot be tested immediately, they should be stored at 2...4°C and brought to room temperature (15...25°C) before testing. • Collect specimen in clean, well rinsed containers, free of detergents. Do not add...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98244bm06